Was it tuned differently before?
Maybe the slot at the nut is too low. Compare its string height at the nut or first fret to the other strings.
You could also have a bad machine head at that string.
It is the same when I tune to E. So far I have tried two .52s and a .50 they all had the problem.
Headstock to tuners aren't the best but all the other strings tune fine with it and I can hear the wavy......MORE »
Thanks for the advice Pushead.
I am tuning with a headstock tuner. I let my friend borrow my pedal. So no pickup required.
The low E is the only string with the problem. My low string is .52 and I......MORE »