The ESP Guitar Company

So the open E is flat, but every other note on the E string is in tune?

How is the tuning before you clamp? Low E is in tune, and every other note is sharp?

Nope before I clamp everything is perfect. After I clamp open string is flat, everything else is perfect.

How flat is it going? Just a little, or a substantial amount? If you bring the open string back into tune using the fine tuners on the bridge, is all well, or is it pushing the rest of the notes on the string sharp?

How hard are you clamping? Any change on the A string at all?

Sorry for the barrage of questions, I've just never heard of this before. Typically my Floyds will go a touch sharp after clamping.

It's not much 5-7 cents.

If I correct it everything went sharp. So intonation between open string and and every fretted string is messed up. I clamp it that way, that my allen wrenge don't move more with light pressure. 

I assume , that clamping the strings, cause any way, that the e string is lifted up...  So so string length is open just a little longer .

Can you tweak the intonation on that string? Intonation on a Floyd is tricky sometimes. I have a tool that makes it easier to do while keeping the string on.

https://www.amazon.com/Allparts-Floyd-Rose-Intonation-Tool/dp/B004MN9746